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Part:BBa_I716301:Design

Designed by: vincent parker   Group: iGEM07_Berkeley_UC   (2007-06-27)

otsA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1456
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1456
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1456
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1456
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2543


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

PCR otsAF/otsAIR on otsA (413 bp, gp=A) PCR otsAIF/otsAR on otsA (1077 bp, gp=B)

PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI) Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L) Product pBca9145-BBa I716301

otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC

References